Takeuchi Masaki    Department   Kawasaki University of Medical Welfare  ,    Position   Associate Professor
Language	English
Title	Mechanical conditions of nesprin-mediated nucleus-actin filament bindings affect polarized elongation of fibroblasts induced by cyclic stretching
Conference	8th World Congress of Biomechanics
Conference Type	International society and overseas society
Presentation Type	Speech
Lecture Type	Panelist at Symposium/Workshop (Applied)
Publisher and common publisher	◎Naoya Sakamoto, Mai Ogawa, Kiyomi Sadamoto, Masaki Takeuchi, Noriyuki Kataoka
Date	2018/07/10
Venue (city and name of the country)	Dublin, Ireland
Summary	Mechanical signals from external environment of a cell are transmitted to the nucleus from cytoskeletons through LINC (linker of nucleoskeleton and cytoskeleton) complex and are believed to play an important role in cellular morphological and functional responses to stimuli from environments. LINC complex are formed by nesprins and SUN proteins at the nuclear envelope, and nesprin-1 and nesprin-2 are believed to directly bind to actin filament. Previous studies have reported that knockdown of nesprin-1causes suppression of cellular morphological responses to mechanical stimuli, indicating that the nucleus-actin filament bindings are crucial to the morphological responses of cells to mechanical stimuli. To further address the detailed roles of nesprin-medicated nucleus-actin filament bindings, we explored the morphological changes of nesprin-1/-2-depleted fibroblasts exposed to cyclic stretching.