Kiyoshi Miura    Department   Kawasaki University of Medical Welfare  ,    Position   Instructor
Article types	原著
Language	English
Peer review	Peer reviewed
Title	Measurement of changes in CO2 generated from propionate in Caenorhabditis elegans using novel 13CO2 gas analysis
Journal	Formal name：Bioscience, Biotechnology, and Biochemistry
Domestic / Foregin	Foregin
Publisher	Bioscience, Biotechnology, and Biochemistry
Volume, Issue, Page	86(7),pp.884-894
Total page number	11
Author and coauthor	Kiyoshi Miura, Hiroki Yamaguchi, Keita Miyata, Tomihiro Miyada
Authorship	Lead author,Corresponding author
Publication date	2022/04
Summary	Caenorhabditis elegans contains two pathways for propionate metabolism, the vitamin B12-dependent and shunt pathways, which are similar to those in humans. In this study, we monitored the changes in propionate metabolism in the whole body of C. elegans using novel 13CO2 gas analysis. We measured the increase in 13CO2 excreted in the air after administering [1-13C]-labeled propionate ([13C]propionate) to the worms. The 13CO2 generated from [13C]propionate in C. elegans increased in a dose-dependent manner, reaching a maximum at 48 h. Enhanced expression of propionate metabolism-related genes was observed after administration of propionate. Knockdown of mmcm-1, which encodes the rate-limiting enzyme of the propionate metabolism, using RNAi reduced 13CO2 excretion. Thus, 13CO2 gas analysis could be confirmed 13CO2 excretion associated with changes in the metabolism of [13C]propionate in nematodes. This analysis can contribute to further understanding of the physiological effects of short-chain fatty acids.