Kouji Yasuyama    Department   Kawasaki University of Medical Welfare  ,    Position   Professor with Special Assignment
Article types	原著
Language	English
Peer review	Peer reviewed
Title	Synaptic connections of cholonergic antennal lobe relay neurons innervating the lateral horn neuropile in the brain of Drosophila melanogaster.
Journal	Formal name：The Journal of Comparative Neurology Abbreviation：J. Comp. Neurol ISSN code：00219967
Volume, Issue, Page	466(3),pp.299-315
Author and coauthor	Yasuyama Kouji, Meinertzhagen Ian A, Schürmann Friedrich-Wilhelm
Authorship	Lead author
Publication date	2003/11
Summary	Presumed cholinergic projection neurons (PNs) in the brain of the fruit fly Drosophila melanogaster, immunoreactive to choline acetyltransferase (ChAT), convey olfactory information between the primary sensory antennal lobe neuropile and the mushroom body calyces, and finally terminate in the lateral horn (LH) neuropile. The texture and synaptic connections of ChAT PNs in the LH and, comparatively, in the smaller mushroom body calyces were investigated by immuno light and electron microscopy. The ChAT PN fibers of the massive inner antennocerebral tract (iACT) extend into all portions of the LH, distributing in a nonrandom fashion. Immunoreactive boutons accumulate in the lateral margins of the LH, whereas the more proximal LH exhibits less intense immunolabeling. Boutons with divergent presynaptic sites, unlabeled as well as ChAT-immunoreactive, appear to be the preponderant mode of synaptic input throughout the LH. Synapses of ChAT-labeled fibers appear predominantly as divergent synaptic boutons (diameters 1–3 um), connected to unlabeled postsynaptic profiles, or alternatively as a minority of tiny postsynaptic spines (diameters 0.05–0.5 um) among unlabeled profiles. Together these spines encircle unidentified presynaptic boutons of interneurons which occupy large areas of the LH. Thus, synaptic circuits in the LH differ profoundly from those of the PNs in the mushroom body calyx, where ChAT spines have not been encountered. Synaptic contacts between LH ChAT elements were not observed. The synaptic LH neuropile may serve as an output area for terminals of the ChAT PNs, their presynaptic boutons providing input to noncholinergic relay neurons. The significance of the postsynaptic neurites of the ChAT PNs is discussed; either local or other interneurons might connect the ChAT PNs within the LH, or PNs might receive inputs arising from outside the LH.
DOI	10.1002/cne.10867