ソノダ ユウジ   Yuji Sonoda
  園田 祐治
   所属   川崎医療福祉大学  医療福祉学部 保健看護学科
   職種   教授
論文種別 原著
言語種別 日本語
査読の有無 査読あり
表題 生後早期のマウス肝臓における成熟好中球の細胞死 : 組織学的観察
掲載誌名 正式名:川崎医学会誌
ISSNコード:03865924
巻・号・頁 21(2),71-80頁
著者・共著者 須田, 満寿美, 園田 祐治, 板野 ちか子, 竹本 琢司, 岩月 宏彦, 佐々木 和信
発行年月 1995
概要 Blood cell production in the fetal liver declines in late gestational life, and liver hemopoietic cells disappear shortly after birth. To clarify the fate of hemopoietic cells in the neonatal liver, we examined ICR mouse livers from 0 to 20 days after birth by light and electron microscopy. In the neonatal liver, a large number of granulocytes accumulated in the interlobular and subcapsular connective tissue, and the majority of the granulocytes could be classified as mature neutrophils. Erythroid cells formed erythroblastic islands, and the islands were scattered throughout the liver parenchyma. After three days of age, liver hemopoietic cells including erythroblasts and neutrophils markedly decreased in number. The neutrophils accumulating in the subcapsular and interlobular connective tissue showed various signs of cell death. The major signs appeared in their nuclei ; condensation and margination of chromatin to form peripheral crescents, and nuclear dissolution. Marked distension of the perinuclear cisternae was noted. The peripheral cytoplasm was fragmented, and the condensed cytoplasm contained swollen lysosomal granules. The apoptotic neutrophils were engulfed and removed by phagocytes. Neutrophils with the morphological appearance of apoptosis were seen within liver macrophages, and highly degraded neutrophil constituents were often present. Programmed cell death of neutrophilic granulocytes is considered to characterize the terminal stage of liver hemopoiesis.