Eri Oyanagi    Department   Kawasaki University of Medical Welfare  ,    Position   Assistant Professor
Article types	原著
Language	English
Peer review	Peer reviewed
Title	L-carnitine is essential to beta-oxidation of quarried fatty acid from mitochondrial membrane by PLA(2)
Journal	Formal name：Mol Cell Biochem Abbreviation：Mol Cell Biochem
Volume, Issue, Page	342(1-2),pp.95-100
Author and coauthor	Hiromi Yano, Eri Oyanagi, Yasuko Kato, Yoshiyuki Samejima, Junzo Sasaki J, Kozo Utsumi
Publication date	2010/09
Summary	Mitochondrial beta-oxidation is an important system involved in the energy production of various cells. In this system, the function of L-carnitine is essential for the uptake of fatty acids to mitochondria. However, it is unclear whether or not endogenous respiration, ADP-induced O(2) consumption without substrates, is caused by L-carnitine treatment. In this study, we investigated whether L-carnitine is essential to the beta-oxidation of quarried fatty acids from the mitochondrial membrane by phospholipase A(2) (PLA(2)) using isolated mitochondria from the liver of rats. Intact mitochondria were incubated in a medium containing Pi, CoA and L-carnitine. The effect of L-carnitine treatment on ADP-induced mitochondrial respiration was observed without exogenous respiratory substrate. Increase in mitochondrial respiration was induced by treatment with L-carnitine in a concentration-dependent manner. Treatment with rotenone, a complex I blocker, completely inhibited ADP-induced oxygen consumption even in the presence of L-carnitine. Moreover, the L-carnitine dependent ADP-induced mitochondrial oxygen consumption did not increase when PLA(2) inhibitors were treated before ADP treatment. The L-carnitine-dependent ADP-induced oxygen consumption did contribute to ATP productions but not heat generation via an uncoupling system. These results suggest that L-carnitine might be essential to the beta-oxidation of quarried fatty acids from the mitochondrial membrane by PLA(2).