フクシマ　ヤスヒロ   Fukushima Yasuhiro   福島　康弘    所属   川崎医療福祉大学  医療福祉学部 医療福祉学科    職種   教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	The dependence of acetylcholine on dynamic changes in the membrane potential and an action potential during spike timing‐dependent plasticity induction in the hippocampus
掲載誌名	正式名：European Journal of Neuroscience
掲載区分	国外
巻・号・頁	56(11),pp.5972-5986
著者・共著者	Eriko Sugisaki, Yasuhiro Fukushima, Naoki Nakajima, Takeshi Aihara
担当区分	2nd著者
発行年月	2022/10/05
概要	The hippocampus is an important area for memory encoding and retrieval and is the location of spike timing-dependent plasticity (STDP), a basic phenomenon of learning and memory. STDP is facilitated if acetylcholine (ACh) is released from cholinergic neurons during attentional processes. However, it is unclear how ACh influences postsynaptic changes during STDP induction and determines the STDP magnitude. To address these issues, we obtained patch clamp recordings from CA1 pyramidal neurons to evaluate the postsynaptic changes during stimuli injection in Schaffer collaterals by quantifying baseline amplitudes (i.e., the lowest values elicited by paired pulses comprising STDP stimuli) and action potentials. The results showed that baseline amplitudes were elevated if eserine was applied in the presence of picrotoxin. In addition, muscarinic ACh receptors (mAChRs) contributed more to the baseline amplitude elevation than nicotinic AChRs (nAChRs). Moreover, the magnitude of the STDP depended on the magnitude of the baseline amplitude. However, in the absence of picrotoxin, baseline amplitudes were balanced, regardless of the ACh concentration, resulting in a similar magnitude of the STDP, except under the nAChR alone-activated condition, which showed a larger STDP and lower baseline amplitude induction. This was due to broadened widths of action potentials. These results suggest that activation of mAChRs and nAChRs, which are effective for baseline amplitudes and action potentials, respectively, plays an important role in postsynaptic changes during memory consolidation.