Akira Yamauchi    Department   Kawasaki Medical School  Kawasaki Medical School, Department of Biochemistry,    Position   Professor
Language	English
Title	The Effect of ROS on the chemotaxis of Inflammatory cells
Conference	Con Bio 2017
Conference Type	Nationwide Conferences
Presentation Type	Poster notice
Lecture Type	General
Publisher and common publisher	Akira Yamauchi Shuichiro Okamoto, Masumi Itadani, Chikage Kawai, Futoshi Kuribayashi
Date	2017/12/06
Venue (city and name of the country)	Kobe
Summary	[Objective] Phagocytes, such as macrophages and neutrophils, play an important role in host defense and migrate toward a focus of infection accompanied by inflammation. This phagocytes' directional migration is altered by various kinds of cytokines and reactive oxygen species (ROS), however, the mechanism how the cells are influenced by the cytokines and ROS is unknown. In this study, we focus on elucidating the relationship between cellular chemotaxis and reactive oxygen species (ROS) at the micro-environment in inflammation. [Methods] Human myeloid leukemia PLB985 (WT PLB) cells and their mutant (X-CGD PLB) cells that defect the superoxide producing enzyme NADPH oxidase subunit gp91phox (NOX2) were used. After differentiation with 0.65% dimethylformamide (DMF), the maturation of the cells was evaluated by checking the expression of NADPH oxidase subunits expression and the chemotaxis of the cells towards fMLP was compared by utilizing TAXIScan method. In addition, as a representative of non-immune cells, BxPC3 cells (pancreatic cancer cells) were also used for chemotaxis assay with hydrogen peroxide. [Results] Both WT PLB and X-CGD PLB cells were differentiated to mature cells with 0.65% DMF and the expression of NADPH oxidase subunits was increased, however, mature X-CGD PLB cells showed more activation in chemotaxis than WT PLB cells. In non-immune BxPC3 cells, hydrogen peroxide suppressed the chemotaxis towards serum in a dose dependent manner. [Discussion] The data above indicate that ROS suppress the chemotaxis, both in myeloid cells and cancer cells. The mechanism that ROS inhibits phagocytes chemotaxis in detail is still unknown and needs to be elucidated.