所属 川崎医科大学 医学部 基礎医学 生化学 職種 教授
|発表タイトル||Formyl peptides and chemokines augment neutrophil accumulation at the inflammation sites whereas lipid mediators support the recruitment.|
|会議名||Annual Meeting of The Japanese Society for Immunology, 2013|
|主催者||The Japanese Society for Immunology|
|Chiba (Makuhari), Japan|
|学会抄録||Proceedings of the Japanese Society for Immunology 42,225 2013|
|概要||Cell-mobility analysis device TAXIScan enables to acquire a large amount of information based on imaging technology, by employing a small number of cells. Using the device, we previously reported that the pattern of neutrophil chemotaxis toward N-formyl-methionyl-leucyl-phenylalanine (fMLP) or interleukin(IL)-8/CXCL8 was directional and rapid far more than that toward lipid mediators such as leukotoriene(LT)B4 and platelet activating factor (PAF). The difference was correlated to the morphology during migration, namely the shape of neutrophils migrating toward fMLP or IL-8/CXCL8 was stable and showed single lamellipodia, which could be observed using higher-magnification-lense. In contrast, the shape of neutrophils migrating toward LTB4 or PAF was unstable, and exhibited multiple directionality and multiple pseudopods.
In the current study, we analyze the effect of co-exiting another attractant on neutrophil migration, which mimics neutrophil accumulation at the inflammation sites. We show that addition of a low concentration of fMLP or IL-8/CXCL8 (1 nM) to LTB4 or PAF induced maximum chemotactic response. On the other hand, any concentrations of LTB4 or PAF up to 100 nM did not influence neutrophil migration toward 10 nM fMLP or IL-8/CXCL8. The results suggest that formyl peptides and chemokines augment neutrophil accumulation at the inflammation sites whereas lipid mediators support the recruitment.
(Non-member contributor; Mikako Degawa-Yamauchi, Futoshi Kuribyashi, Shiro Kanegasaki, and Tomoko Tsuchiya)