Akira Yamauchi    Department   Kawasaki Medical School  Kawasaki Medical School, Department of Biochemistry,    Position   Professor
Language	English
Title	The robust directionality in neutrophil chemotaxis to fMLP: an optical approach to the mechanism.
Conference	The 85th annual meeting of Japanese Society for Bacteriology
Conference Type	Nationwide Conferences
Presentation Type	Poster notice
Lecture Type	General
Publisher and common publisher	Akira Yamauchi, Shiro Kanegasaki, and Futoshi Kuribayashi
Date	2012/03/27
Venue (city and name of the country)	Nagasaki, Japan
Society abstract	Japanese journal of Bacteriology 67(1),155 2012
Summary	Purpose: A real-time cell mobility analysis device “TAXIScan” enables to acquire a large amount of information based on imaging technology employing a small number of cells. Previously we reported that the pattern of neutrophil chemotaxis to a synthetic peptide N-formyl-methionyl-leucyl-phenylalanine (fMLP) originally isolated from bacteria is more directional and rapid than internal chemoattractants such as platelet activating factor (PAF) in vitro. We have developed TAXIScan-FL equipped with a fluorescence detection system and with a high power objective lens, and used it to investigate the difference of neutrophil chemotaxis patterns in detail. Methods: Human neutrophils were isolated from peripheral blood. The chemotaxis was analyzed with EZ-TAXIScan and the high-end model TAXIScan-FL. Results: In the higher–magnification-view, the shape of neutrophils migrating to fMLP was stable with a single and wide lamellipodia. In contrast, the shape of neutrophils migrating to PAF was unstable, and exhibited multiple directionality and multiple pseudopods. Discussion: The data presented here indicate that the robust directionality of neutrophil chemotaxis to fMLP with a consistency of directionality is maintained by keeping shape of cells with a single and wide lamellipodia. Molecular mechanism is now under investigation.