ヤマウチ　アキラ   Akira Yamauchi   山内　明    所属   川崎医科大学  医学部 基礎医学　生化学    職種   教授
言語種別	英語
発表タイトル	The robust directionality in neutrophil chemotaxis to fMLP: an optical approach to the mechanism.
会議名	The 85th annual meeting of Japanese Society for Bacteriology
学会区分	全国規模の学会
発表形式	ポスター掲示
講演区分	一般
発表者・共同発表者	Akira Yamauchi, Shiro Kanegasaki, and Futoshi Kuribayashi
発表年月日	2012/03/27
開催地 （都市, 国名）	Nagasaki, Japan
学会抄録	Japanese journal of Bacteriology 67(1),155 2012
概要	Purpose: A real-time cell mobility analysis device “TAXIScan” enables to acquire a large amount of information based on imaging technology employing a small number of cells. Previously we reported that the pattern of neutrophil chemotaxis to a synthetic peptide N-formyl-methionyl-leucyl-phenylalanine (fMLP) originally isolated from bacteria is more directional and rapid than internal chemoattractants such as platelet activating factor (PAF) in vitro. We have developed TAXIScan-FL equipped with a fluorescence detection system and with a high power objective lens, and used it to investigate the difference of neutrophil chemotaxis patterns in detail. Methods: Human neutrophils were isolated from peripheral blood. The chemotaxis was analyzed with EZ-TAXIScan and the high-end model TAXIScan-FL. Results: In the higher–magnification-view, the shape of neutrophils migrating to fMLP was stable with a single and wide lamellipodia. In contrast, the shape of neutrophils migrating to PAF was unstable, and exhibited multiple directionality and multiple pseudopods. Discussion: The data presented here indicate that the robust directionality of neutrophil chemotaxis to fMLP with a consistency of directionality is maintained by keeping shape of cells with a single and wide lamellipodia. Molecular mechanism is now under investigation.