所属 川崎医科大学 医学部 基礎医学 生化学 職種 教授
|発表タイトル||The robust directionality in neutrophil chemotaxis to fMLP: an optical approach to the mechanism.|
|会議名||The 85th annual meeting of Japanese Society for Bacteriology|
|発表者・共同発表者||Akira Yamauchi, Shiro Kanegasaki, and Futoshi Kuribayashi|
|学会抄録||Japanese journal of Bacteriology 67(1),155 2012|
|概要||Purpose: A real-time cell mobility analysis device “TAXIScan” enables to acquire a large amount of information based on imaging technology employing a small number of cells. Previously we reported that the pattern of neutrophil chemotaxis to a synthetic peptide N-formyl-methionyl-leucyl-phenylalanine (fMLP) originally isolated from bacteria is more directional and rapid than internal chemoattractants such as platelet activating factor (PAF) in vitro. We have developed TAXIScan-FL equipped with a fluorescence detection system and with a high power objective lens, and used it to investigate the difference of neutrophil chemotaxis patterns in detail.
Methods: Human neutrophils were isolated from peripheral blood. The chemotaxis was analyzed with EZ-TAXIScan and the high-end model TAXIScan-FL.
Results: In the higher–magnification-view, the shape of neutrophils migrating to fMLP was stable with a single and wide lamellipodia. In contrast, the shape of neutrophils migrating to PAF was unstable, and exhibited multiple directionality and multiple pseudopods.
Discussion: The data presented here indicate that the robust directionality of neutrophil chemotaxis to fMLP with a consistency of directionality is maintained by keeping shape of cells with a single and wide lamellipodia. Molecular mechanism is now under investigation.