ニシマツ シンイチロウ
Shinichiro Nishimatsu
西松 伸一郎 所属 川崎医科大学 医学部 一般教養 自然科学 職種 教授 |
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論文種別 | 原著 |
言語種別 | 英語 |
査読の有無 | 査読あり |
表題 | A Wnt3a variant participates in chick apical ectodermal ridge formation: distinct biological activities of Wnt3a splice variants in chick limb development. |
掲載誌名 | 正式名:Development, growth & differentiation 略 称:Dev Growth Differ ISSNコード:00121592/1440169X |
巻・号・頁 | 49(6),pp.493-501 |
著者・共著者 | Narita Tomohiro, Nishimatsu Shin-ichiro, Wada Naoyuki, Nohno Tsutomu |
担当区分 | 2nd著者 |
発行年月 | 2007/08 |
概要 | Wnt/beta-catenin signaling is involved in the formation of the apical ectodermal ridge (AER) during vertebrate limb development. Although Wnt3a is a potent ligand for chick AER formation, whether chick Wnt3a can induce Fgf8 expression in chick embryos is unclear and the Wnt ligand involved in chick AER formation remains unknown. Here, we examined whether another Wnt3a isoform is expressed in the AER, and whether Wnt3 contributes to AER formation in chick as well as mouse embryos. We found that chick Wnt3 was not expressed in the presumptive limb ectoderm at the early stages of AER formation. Using 5'-rapid amplification of cDNA ends, we isolated another chick Wnt3a transcript. This novel variant, Wnt3a variant 2, induced Fgf8 in the limb ectoderm and activated the beta-catenin pathway in vivo and in vitro. These data showed that Wnt3a variant 2 is an active form of chick Wnt3a that regulates chick AER formation. |
DOI | 10.1111/j.1440-169X.2007.00938.x |
文献番号 | 17488271 |