Shinichiro Nishimatsu    Department   Kawasaki Medical School  Kawasaki Medical School, Department of Natural Sciences,    Position   Professor
Article types	原著
Language	English
Peer review	Peer reviewed
Title	Wnt10a is involved in AER formation during chick limb development.
Journal	Formal name：Developmental dynamics : an official publication of the American Association of Anatomists Abbreviation：Dev Dyn ISSN code：10588388
Volume, Issue, Page	233(2),pp.282-287
Author and coauthor	Narita Tomohiro, Sasaoka Shunsuke, Udagawa Kiyoshi, Ohyama Takahiro, Wada Naoyuki, Nishimatsu Shin-ichiro, Takada Shinji, Nohno Tsutomu
Publication date	2005/06
Summary	The apical ectodermal ridge (AER) is indispensable for vertebrate limb development and requires Wnt/beta-catenin signaling for induction and maintenance. We report identification and involvement of Wnt10a in AER formation during chick limb development. Chicken Wnt10a has 82% identity with mouse Wnt10a in the amino acid sequence. The Wnt10a gene was expressed broadly in the surface ectoderm from as early as stage 10. By stage 15, the expression was restricted to the surface ectoderm overlying the lateral plate mesoderm. Wnt10a expression became intensified in the presumptive limb ectoderm during AER formation, and subsequently intense expression signals persisted in the AER. Wnt10a misexpression led to ectopic Fgf8 expression in the developing limb ectoderm and induced translocation of beta-catenin in chick embryo fibroblasts. These results suggest that Wnt10a is involved in AER formation in the chick limb bud through the Wnt/beta-catenin signaling pathway.
DOI	10.1002/dvdy.20321
Document No.	15789446