ニシマツ　シンイチロウ   Shinichiro Nishimatsu   西松　伸一郎    所属   川崎医科大学  医学部 一般教養　自然科学    職種   教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Cytoplasmic and molecular reconstruction of Xenopus embryos: Synergy of dorsalizing and endo-mesodermalizing determinants drives early axial patterning.
掲載誌名	正式名：Development 略　 称：Development ISSNコード：09501991
巻・号・頁	131(5),pp.1135-1144
著者・共著者	Katsumoto Keiichi, Arikawa Tomohiro, Doi Jun-Ya, Fujii Hidefumi, Nishimatsu Shin-Ichiro, Sakai Masao
発行年月	2004/05
概要	Ablation of vegetal cytoplasm from newly fertilized Xenopus eggs results in the development of permanent blastula-type embryos (PBEs). PBEs cleave normally and develop into a very simple tissue consisting only of atypical epidermis. We tried to restore complete embryonic development in PBEs by cytoplasmic transplantation or by mRNA injection. We show a two-step reconstruction of the body plan. In the first step, PBEs injected with either marginal cytoplasm or synthetic VegT RNA restored gastrulation and mesoderm formation, but not axial patterning. Injection of Xwnt8 mRNA (acting upstream of beta-catenin and thus substitutes for the dorsal determinant) did not restore axial development in PBEs. Simultaneous injections of Xwnt8 and VegT into PBEs resulted in dorsal axis development, showing the synergy of these molecules in axial development. These results suggest that the mixing of two cytoplasmic determinants, i.e. the dorsal determinant in the vegetal pole and the endo-mesodermal determinant in the whole vegetal half, triggers the early axial developmental process in Xenopus embryos.
DOI	10.1242/dev.01015
文献番号	14973279