ニシマツ シンイチロウ
Shinichiro Nishimatsu
西松 伸一郎 所属 川崎医科大学 医学部 一般教養 自然科学 職種 教授 |
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論文種別 | 原著 |
言語種別 | 英語 |
査読の有無 | 査読あり |
表題 | Biochemical properties of amphibian bone morphogenetic protein-4 expressed in CHO cells. |
掲載誌名 | 正式名:The Biochemical journal 略 称:Biochem J ISSNコード:02646021/14708728 |
巻・号・頁 | 291(2),pp.413-417 |
著者・共著者 | Suzuki Atsushi, Nishimatsu Shin-ichiro, Shoda Akihito, Takebayashi Kimiko, Murakami Kazuo, Ueno Naoto |
担当区分 | 2nd著者 |
発行年月 | 1993/04 |
概要 | The biochemical properties of recombinant amphibian bone morphogenetic protein-4 (BMP-4), the cDNA of which has been cloned recently by screening of a Xenopus cDNA library, was characterized. The protein was expressed by the transfection of Chinese hamster ovary (CHO) cells with the cDNA cloned into expression vectors bearing a cytomegalovirus promoter or a simian virus 40 promoter. Northern-blot analysis showed that the latter vector was more efficient for Xenopus BMP-4 expression. Specific antiserum against Xenopus BMP-4 peptide demonstrated that the protein is synthesized as a large precursor, processed to the mature form and then secreted from the cells as a homodimer. Analysis of the biological activity in the conditioned medium revealed that Xenopus BMP-4 has a potent alkaline phosphatase-inducing activity on mouse osteoblastic cells. |
文献番号 | 8387268 |