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Shinichiro Nishimatsu
Department Kawasaki Medical School Kawasaki Medical School, Department of Natural Sciences, Position Professor |
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| Article types | 原著 |
| Language | English |
| Peer review | Peer reviewed |
| Title | Expression of thymosin beta4 gene during Xenopus laevis embryogenesis. |
| Journal | Formal name：Biochemical and biophysical research communications Abbreviation：Biochem Biophys Res Commun ISSN code：0006291X/10902104 |
| Volume, Issue, Page | 184(15),pp.93-99 |
| Author and coauthor | Yamamoto Masaya, Shoda Akihito, Minamino Naoto, Matsuo Hisayuki, Nishimatsu Shin-ichiro, Ueno Naoto, Murakami Kazuo |
| Publication date | 1992/04 |
| Summary | In order to investigate the molecular events which take place during gastrulation, extracts from developing Xenopus embryos were analyzed for temporal peptide distribution by high performance liquid chromatography. One peptide peak which became increasingly dominant after gastrulation was purified and partially characterized. The amino acid sequence of enzymic digests showed the peptide is extremely similar to mammalian thymosin beta4. The peptide was capable of binding actin monomers like mammalian counterparts. Cloning of the Xenopus thymosin beta4 cDNA showed that only three amino acid substitutions occurred between amphibian and bovine. Northern blot analysis revealed the mRNA is maternally present at a low level and the transcript becomes abundant after gastrulation, supporting the distribution of the peptide. |
| DOI | 10.1016/0006-291X(92)91162-J |
| Document No. | 1567461 |