Shinichiro Nishimatsu    Department   Kawasaki Medical School  Kawasaki Medical School, Department of Natural Sciences,    Position   Professor
Article types	原著
Language	English
Peer review	Peer reviewed
Title	Involvement of frizzled-10 in Wnt-7a signaling during chick limb development.
Journal	Formal name：Development, growth & differentiation Abbreviation：Dev Growth Differ ISSN code：00121592/1440169X
Volume, Issue, Page	42(6),pp.561-569
Author and coauthor	Kawakami Yasuhiko, Wada Naoyuki, Nishimatsu Shin-ichiro, Nohno Tsutomu
Publication date	2000/12
Summary	The dorsal ectoderm of the limb bud is known to regulate anterior-posterior patterning as well as dorsalventral patterning during vertebrate limb morphogenesis. Wnt-7a, expressed in the dorsal ectoderm, encodes a key molecule implicated in these events. In the present study, chicken frizzled-10 (Fz-10) encoding a Wnt receptor was used to study mechanisms of Wnt-7a signaling during chick limb patterning, because its expression is restricted to the posterior-distal region of the dorsal limb bud. Fz-10 transcripts colocalize with Sonic hedgehog (Shh) in the dorsal side of stages 18-23 chick limb buds. It was demonstrated that Fz-10 interacts with Wnt-7a to induce synergistically the expression of Wnt-responsive genes, such as Siamois and Xnr3, in Xenopus animal cap assays. In the chick limb bud, Fz-10 expression is regulated by Shh and a signal from the dorsal ectoderm, presumably Wnt-7a, but not by signals from the apical ectodermal ridge. These results suggest that Fz-10 acts as a receptor for Wnt-7a and has a positive effect on Shh expression in the chick limb bud.
DOI	10.1046/j.1440-169X.2000.00545.x
Document No.	11142678