Hideho Wada    Department   Kawasaki Medical School  Kawasaki Medical School, Department of Hematology,    Position   Professor
Article types	原著
Language	English
Peer review	Peer reviewed
Title	Screening for human immunodeficiency virus using a newly developed fourth generation lateral flow immunochromatography assay
Journal	Formal name：Journal of Virological Methods Abbreviation：J.Virol.Methods ISSN code：0166-0934
Domestic / Foregin	Foregin
Publisher	ELSEVIER
Volume, Issue, Page	274,pp.1-5
Author and coauthor	Itsuhiro Nakagiri, Taizo Tasaka, Miki Okai, Fukue Nakai, Ryoko Bunya, Satomi Nagai, Tomoko Yoshida, Hirotoshi Tokunaga, Eisei Kondo, Hideho Wada
Authorship	Last author
Publication date	2019/12
Summary	We screened 17,373 preoperative out patient blood samples using 4th generation lateral flow immunochromatography Daina Screen HIV Combo assay for simultaneously detecting anti-HIV-1/2 and HIV-1 p24 antigen. Of the samples tested, 24 were positive for HIV-1 p24 antigen and 49 for HIV-1/2 antibody. Of the 49, samples, 36 were WB and HIV-1 RNA negative, 10 were WB and HIV-1 RNA positive, and 3 were WB positive, HIV-1 RNA negative, and in-house HIV-1 proviral DNA positive. RT-PCR revealed that of the 24 samples that were p24 antigen positive, one sample was HIV-1 RNA positive, which was reconfirmed using an in-house HIV-1 provirus DNA assay. From the 17,300 HIV-1 p24 antigen and anti-HIV-1/2 negative samples, pools containing 10 negative samples each were tested for HIV-1 by RT-PCR; all results were negative. The Daina Screen HIV Combo assay had a sensitivity and specificity of 100% and 99.7%, respectively, which sufficiently detected HIV infection in the cohort.
DOI	10.1016/j.jviromet.2019.113746
Document No.	113746