トクトミ　トモハル   Tomoharu Tokutomi   徳富　智明    所属   川崎医科大学  医学部 臨床医学　小児科学    職種   特任教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Intravenous immunoglobulin therapy induces neutrophil apoptosis in Kawasaki disease.
掲載誌名	正式名：Clinical immunology (Orlando, Fla.) ISSNコード：1521-6616
掲載区分	国外
出版社	ACADEMIC PRESS INC ELSEVIER SCIENCE
巻・号・頁	103(2),pp.161-8
著者・共著者	Hiroshi Tsujimoto,Seiichiro Takeshita,Keigo Nakatani,Youichi Kawamura,Tomoharu Tokutomi,Isao Sekine
発行年月	2002/05
概要	To investigate the effect of high-dose intravenous immunoglobulin (IVIg) on neutrophil apoptosis in Kawasaki disease (KD), we studied the in vitro spontaneous and IVIg-induced apoptosis of neutrophils by analyzing a proportion of annexin V-positive cells and cells with fragmented DNA. The mean number of peripheral neutrophils in the post-IVIg phase decreased significantly (P < 0.01) compared with that in the pre-IVIg phase. The mean proportion of spontaneous apoptotic neutrophils in the post-IVIg phase was significantly higher (P < 0.01) than that in the pre-IVIg phase, and there was a significantly positive correlation (P < 0.01) with the reduction ratio of the circulating neutrophil counts from the pre-IVIg through the post-IVIg phases. IVIg induced a dose-dependent increase in the proportion of apoptotic neutrophils in the pre-IVIg phase. As a result, the present study demonstrated a novel action in which high-dose IVIg therapy decreased the number of circulating neutrophils by accelerating their apoptosis in KD. (C) 2002 Elsevier Science (USA).
DOI	10.1006/clim.2002.5209
PMID	12027421