|
Seigo Terawaki
Department Kawasaki Medical School Kawasaki Medical School, Department of Molecular and Genetic Medicine, Position Assistant Professor with Special Assignment |
|
| Article types | 原著 |
| Language | English |
| Peer review | Peer reviewed |
| Title | RUFY4 exists as two translationally regulated isoforms, that localize to the mitochondrion in activated macrophages. |
| Journal | Formal name：Royal Society open science Abbreviation：R Soc Open Sci ISSN code：20545703/20545703 |
| Domestic / Foregin | Foregin |
| Volume, Issue, Page | 8(7),pp.202333 |
| Author and coauthor | Valečka Jan, Camosseto Voahirana, McEwan David G, Terawaki Seigo, Liu Zhuangzhuang, Strock Eva, Almeida Catarina R, Su Bing, Dikic Ivan, Liang Yinming, Gatti Evelina, Pierre Philippe |
| Publication date | 2021/07 |
| Summary | We report here that RUFY4, a newly characterized member of the 'RUN and FYVE domain-containing' family of proteins previously associated with autophagy enhancement, is highly expressed in alveolar macrophages (AM). We show that RUFY4 interacts with mitochondria upon stimulation by microbial-associated molecular patterns of AM and dendritic cells. RUFY4 interaction with mitochondria and other organelles is dependent on a previously uncharacterized OmpH domain located immediately upstream of its C-terminal FYVE domain. Further, we demonstrate that rufy4 messenger RNA can be translated from an alternative translation initiation codon, giving rise to a N-terminally truncated form of the molecule lacking most of its RUN domain and with enhanced potential for its interaction with mitochondria. Our observations point towards a role of RUFY4 in selective mitochondria clearance in activated phagocytes. |
| DOI | 10.1098/rsos.202333 |
| PMID | 34295519 |