テラワキ セイゴウ   Seigo Terawaki
  寺脇 正剛
   所属   川崎医科大学  医学部 基礎医学 分子遺伝医学
   職種   特任講師
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 RUN and FYVE domain-containing protein 4 enhances autophagy and lysosome tethering in response to Interleukin-4.
掲載誌名 正式名:The Journal of cell biology
略  称:J Cell Biol
ISSNコード:15408140/00219525
掲載区分国外
巻・号・頁 210(7),1133-1152頁
著者・共著者 Terawaki Seigo, Camosseto Voahirana, Prete Francesca, Wenger Till, Papadopoulos Alexia, Rondeau Christiane, Combes Alexis, Rodriguez Rodrigues Christian, Vu Manh Thien-Phong, Fallet Mathieu, English Luc, Santamaria Rodrigo, Soares Ana R, Weil Tobias, Hammad Hamida, Desjardins Michel, Gorvel Jean-Pierre, Santos Manuel A S, Gatti Evelina, Pierre Philippe
発行年月 2015/09
概要 Autophagy is a key degradative pathway coordinated by external cues, including starvation, oxidative stress, or pathogen detection. Rare are the molecules known to contribute mechanistically to the regulation of autophagy and expressed specifically in particular environmental contexts or in distinct cell types. Here, we unravel the role of RUN and FYVE domain-containing protein 4 (RUFY4) as a positive molecular regulator of macroautophagy in primary dendritic cells (DCs). We show that exposure to interleukin-4 (IL-4) during DC differentiation enhances autophagy flux through mTORC1 regulation and RUFY4 induction, which in turn actively promote LC3 degradation, Syntaxin 17-positive autophagosome formation, and lysosome tethering. Enhanced autophagy boosts endogenous antigen presentation by MHC II and allows host control of Brucella abortus replication in IL-4-treated DCs and in RUFY4-expressing cells. RUFY4 is therefore the first molecule characterized to date that promotes autophagy and influences endosome dynamics in a subset of immune cells.
DOI 10.1083/jcb.201501059
PMID 26416964