Sotatsu Tonomura
   Department   Kawasaki Medical School  Kawasaki Medical School, Department of Anatomy,
   Position   Instructor
Article types 原著
Language English
Peer review Peer reviewed
Title Protocol for pressure-clamped patch-clamp recording at the node of Ranvier of rat myelinated nerves
Journal Formal name：STAR Protoc
ISSN code：26661667
Domestic / ForeginForegin
Volume, Issue, Page 2(1),pp.100266
Author and coauthor Kanda, H. Tonomura, S. Dai, Y. Gu, J. G.
Publication date 2021
Summary The patch-clamp recording technique is indispensable for studying ion channel functions of cells but is challenging to apply to the node of Ranvier, a key site where action potentials are conducted along myelinated nerves. We have developed a pressure-clamped patch-clamp recording method applying to the node of Ranvier of rat myelinated nerves. The step-by-step protocol described here allows researchers to apply this approach to study mechanisms underlying saltatory conduction and information processing in myelinated nerves of mammals. For complete information on the generation and use of this protocol, please refer to Kanda et al. (2019).
DOI 10.1016/j.xpro.2020.100266
Document No. 33490982