オノ　シゲキ   Shigeki Ono   小野　成紀    所属   川崎医科大学  医学部 臨床医学　脳神経外科学２    職種   教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Endothelial nitric oxide synthase-11R protein therapy for prevention of cerebral vasospasm in rats: a preliminary report
掲載誌名	正式名：Acta Neurochirurgica Supplement 略　 称：Acta Neurochir Suppl ISSNコード：00651419
巻・号・頁	104,pp.165-167
著者・共著者	Tomoyuki Ogawa, S. Ono, T. Ichikawa, S. Arimitsu, K. Onoda, K. Tokunaga, K. Sugiu, K. Tomizawa, H. Matsui, I. Date
発行年月	2008
概要	Background In one of our studies we found that enhanced green fluorescent protein (EGFP) fused with consecutive 11 arginines (11R), one of the protein transduction domains (PTDs) [1–6, 11], and effectively penetrated into all layers of the rat basilar artery (BA). We examined whether eNOS (140-kDa) fused 11R (11R-eNOS) was also transduced into the BAs and had a positive effect on the attenuation of cerebral vasospasm. Method 11R-eNOS or saline was injected into the cisterna magna of male Sprague-Dawley rats. Two hours after the injection, the BAs were extracted from the rats and transduction efficacy of 11R-eNOS in the BA was evaluated by immunofluorescence staining. To examine the effect of 11R-eNOS on the cerebral arteries exposed to SAH, we measured the post SAH BA diameters six hours after the injection of 11R-eNOS. Findings Immunofluorescent study confirmed the presence of 11R-eNOS protein in the layers of the cerebral arteries in vivo. 11R-eNOS had a positive effect on attenuation of cerebral vasospasm. Conclusions 11R-eNOS was effectively transduced into the walls of the BA. 11R-eNOS inhibited the vasoconstriction after SAH. These results suggest that 11R-eNOS protein therapy has a potential in treating cerebral vasospasm.