ヤマウチ アキラ
  山内 明
   所属   川崎医科大学  医学部 基礎医学 生化学
   職種   教授
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 Human skin melanocyte migration towards stromal cell-derived factor-1a demonstrated by optical real-time cell mobility assay: Modulation of their chemotactic ability by a-melanocyte stimulating hormone
掲載誌名 正式名:Experimental Dermatology
略  称:EXD
ISSNコード:1600-0625
巻・号・頁 22(10),664-667頁
著者・共著者 Akira Yamauchi, Christophe Hadjur, Tadahito Takahashi, Itaru Suzuki, Kunitaka Hirose, Yann F. Mahe.
発行年月 2013/10
概要 To identify potential regulators of normal human melanocyte behaviour, we have developed an in vitro human melanocyte migration assay, using the optically accessible, real-time cell motility assay device TAXIScan. Coating of the glass surface with an extracellular matrix that served as scaffolding molecule was essential to demonstrate efficient melanocyte migration. Among several chemokines tested, stromal cell-derived factor (SDF)-1α/CXCL12 was the most effective driver of human normal skin melanocytes. Incubation of melanocytes with α-melanocyte-stimulating hormone (MSH) before the assay specifically enhanced CXCR4 expression and consequently chemotaxis towards SDF-1α/CXCL12. These results suggest that α-MSH acts on melanocytes to produce melanin as well as stimulates the cells to migrate to the site where they work through CXCR4 up-regulation, which is a new dynamic mode of action of α-MSH on melanocyte physiology.
DOI DOI: 10.1111/exd.12232