Michi Miura    Department   Kawasaki Medical School  Kawasaki Medical School, Department of Microbiology,    Position   Instructor
Article types	原著
Language	English
Peer review	Peer reviewed
Title	The human leukemia virus HTLV-1 alters the structure and transcription of host chromatin in cis.
Journal	Formal name：eLife Abbreviation：Elife ISSN code：2050084X/2050084X
Domestic / Foregin	Foregin
Volume, Issue, Page	7,pp.e36245
Author and coauthor	Melamed Anat, Yaguchi Hiroko, Miura Michi, Witkover Aviva, Fitzgerald Tomas W, Birney Ewan, Bangham Charles RM
Publication date	2018/06
Summary	Chromatin looping controls gene expression by regulating promoter-enhancer contacts, the spread of epigenetic modifications, and the segregation of the genome into transcriptionally active and inactive compartments. We studied the impact on the structure and expression of host chromatin by the human retrovirus HTLV-1. We show that HTLV-1 disrupts host chromatin structure by forming loops between the provirus and the host genome; certain loops depend on the critical chromatin architectural protein CTCF, which we recently discovered binds to the HTLV-1 provirus. We show that the provirus causes two distinct patterns of abnormal transcription of the host genome in cis: bidirectional transcription in the host genome immediately flanking the provirus, and clone-specific transcription in cis at non-contiguous loci up to >300 kb from the integration site. We conclude that HTLV-1 causes insertional mutagenesis up to the megabase range in the host genome in >104 persistently-maintained HTLV-1+ T-cell clones in vivo.
DOI	10.7554/eLife.36245
PMID	29941091