ミウラ ミチ   Michi Miura
  三浦 未知
   所属   川崎医科大学  医学部 基礎医学 微生物学
   職種   助教
Article types 原著
Language English
Peer review Peer reviewed
Title The retrovirus HTLV-1 inserts an ectopic CTCF-binding site into the human genome.
Journal Formal name:Proceedings of the National Academy of Sciences of the United States of America
Abbreviation:Proc Natl Acad Sci U S A
ISSN code:10916490/00278424
Domestic / ForeginForegin
Volume, Issue, Page 113(11),pp.3054-9
Author and coauthor Satou Yorifumi, Miyazato Paola, Ishihara Ko, Yaguchi Hiroko, Melamed Anat, Miura Michi, Fukuda Asami, Nosaka Kisato, Watanabe Takehisa, Rowan Aileen G, Nakao Mitsuyoshi, Bangham Charles R M
Publication date 2016/03
Summary Human T-lymphotropic virus type 1 (HTLV-1) is a retrovirus that causes malignant and inflammatory diseases in ∼10% of infected people. A typical host has between 10(4) and 10(5) clones of HTLV-1-infected T lymphocytes, each clone distinguished by the genomic integration site of the single-copy HTLV-1 provirus. The HTLV-1 bZIP (HBZ) factor gene is constitutively expressed from the minus strand of the provirus, whereas plus-strand expression, required for viral propagation to uninfected cells, is suppressed or intermittent in vivo, allowing escape from host immune surveillance. It remains unknown what regulates this pattern of proviral transcription and latency. Here, we show that CTCF, a key regulator of chromatin structure and function, binds to the provirus at a sharp border in epigenetic modifications in the pX region of the HTLV-1 provirus in T cells naturally infected with HTLV-1. CTCF is a zinc-finger protein that binds to an insulator region in genomic DNA and plays a fundamental role in controlling higher order chromatin structure and gene expression in vertebrate cells. We show that CTCF bound to HTLV-1 acts as an enhancer blocker, regulates HTLV-1 mRNA splicing, and forms long-distance interactions with flanking host chromatin. CTCF-binding sites (CTCF-BSs) have been propagated throughout the genome by transposons in certain primate lineages, but CTCF binding has not previously been described in present-day exogenous retroviruses. The presence of an ectopic CTCF-BS introduced by the retrovirus in tens of thousands of genomic locations has the potential to cause widespread abnormalities in host cell chromatin structure and gene expression.
DOI 10.1073/pnas.1423199113
PMID 26929370