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Michi Miura
Department Kawasaki Medical School Kawasaki Medical School, Department of Microbiology, Position Instructor |
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| Article types | 原著 |
| Language | English |
| Peer review | Peer reviewed |
| Title | Nucleotide-dependent behavior of single molecules of cytoplasmic dynein on microtubules in vitro. |
| Journal | Formal name：FEBS letters Abbreviation：FEBS Lett ISSN code：18733468/00145793 |
| Domestic / Foregin | Foregin |
| Volume, Issue, Page | 584(11),pp.2351-5 |
| Author and coauthor | Miura Michi, Matsubara Aiko, Kobayashi Takuya, Edamatsu Masaki, Toyoshima Yoko Y |
| Authorship | Lead author |
| Publication date | 2010/06 |
| Summary | We visualized the nucleotide-dependent behavior of single molecules of mammalian native cytoplasmic dynein using fragments of dynactin p150 with or without its N-terminal microtubule binding domain. The results indicate that the binding affinity of dynein for microtubules is high in AMP-PNP, middle in ADP or no nucleotide, and low in ADP.Pi conditions. It is also demonstrated that the microtubule binding domain of dynactin p150 maintains the association of dynein with microtubules without altering the motile property of dynein in the weak binding state. In addition, we observed bidirectional movement of dynein in the presence of ATP as well as in ADP/Vi condition, suggesting that the bidirectional movement is driven by diffusion rather than active transport. |
| DOI | 10.1016/j.febslet.2010.04.016 |
| PMID | 20394748 |