Shuya Yano
   Department   Kawasaki Medical School  Kawasaki Medical School, Department of Digestive Surgery,
   Position   Assistant Professor
Article types 原著
Language English
Peer review Peer reviewed
Title Immuno-hyperthermia effected by antibody-conjugated nanoparticles selectively targets and eradicates individual cancer cells.
Journal Formal name：Cell cycle (Georgetown, Tex.)
Abbreviation：Cell Cycle
ISSN code：15514005/15514005
Domestic / ForeginForegin
Volume, Issue, Page 20(13),pp.1221-1230
Author and coauthor Kagawa Tetsuya, Matsumi Yuki, Aono Hiromichi, Ohara Toshiaki, Tazawa Hiroshi, Shigeyasu Kunitoshi, Yano Shuya, Takeda Sho, Komatsu Yasuhiro, Hoffman Robert M, Fujiwara Toshiyoshi, Kishimoto Hiroyuki
Publication date 2021/07
Summary Hyperthermia has been used for cancer therapy for a long period of time, but has shown limited clinical efficacy. Induction-heating hyperthermia using the combination of magnetic nanoparticles (MNPs) and an alternating magnetic field (AMF), termed magnetic hyperthermia (MHT), has previously shown efficacy in an orthotopic mouse model of disseminated gastric cancer. In the present study, superparamagnetic iron oxide nanoparticles (SPIONs), a type of MNP, were conjugated with an anti-HER2 antibody, trastuzumab and termed anti-HER2-antibody-linked SPION nanoparticles (anti-HER2 SPIONs). Anti-HER2 SPIONs selectively targeted HER2-expressing cancer cells co-cultured along with normal fibroblasts and HER2-negative cancer cells and caused apoptosis only in the HER2-expressing individual cancer cells. The results of the present study show proof-of-concept of a novel hyperthermia technology, immuno-MHT for selective cancer therapy, that targets individual cancer cells.Abbreviations: AMF: alternating magnetic field; DDW: double distilled water; DMEM: Dulbecco's Modified Eagle's; Medium; f: frequency; FBS: fetal bovine serum; FITC: Fluorescein isothiocyanate; GFP: green fluorescent protein; H: amplitude; Hsp: heat shock protein; MHT: magnetic hyperthermia; MNPs: magnetic nanoparticles; PI: propidium iodide; RFP: red fluorescent protein; SPION: superparamagnetic iron oxide (Fe3O4) nanoparticle.
DOI 10.1080/15384101.2021.1915604
PMID 34148497