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サカモト ユウマ
Yuma Sakamoto
坂本 祐真 所属 川崎医科大学 医学部 基礎医学 免疫学 職種 助教 |
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| 論文種別 | 原著 |
| 言語種別 | 英語 |
| 査読の有無 | 査読あり |
| 表題 | Next-generation sequencing assay in salivary gland cytology: A pilot study. |
| 掲載誌名 | 正式名：Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology 略 称：J Oral Pathol Med ISSNコード：16000714/09042512 |
| 掲載区分 | 国外 |
| 巻・号・頁 | 49(10),pp.1037-1043 |
| 著者・共著者 | Shuhei Yamamoto, Yuma Sakamoto, Satsuki Nakano, Keiichiro Fujii, Kaori Ueda, Yoshihide Okumura, Kana Tsuda, Ayako Masaki, Daisuke Kawakita, Takayuki Murase, Hiroshi Inagaki |
| 発行年月 | 2020/11 |
| 概要 | BACKGROUND:Preoperative diagnosis of salivary gland tumors (SGTs) by fine-needle aspiration (FNA) cytology is challenging. Next-generation sequencing (NGS)-based assays for somatic mutations have a great advantage in that a large number of genes can be analyzed simultaneously. Although NGS may have an enormous diagnostic potential in cytology, to our knowledge, the significance of NGS in SGT cytology remains to be clarified.METHODS:In this pilot study, we retrospectively examined 32 frozen SGT samples obtained at surgery (14 malignant and 18 benign). After the stored frozen tumor tissues were thawed, aspirate samples were obtained using 22-gauge needles and subjected to smear tumor samples and to DNA extraction for an NGS assay employing the Illumina AmpliSeq Cancer Hotspot Panel v2. The results were correlated to preoperative cytological diagnosis.RESULTS:The preoperative diagnoses obtained by FNA cytology included 23 negative lesions (no malignancy in 6 and benign tumor in 17) and nine positive lesions (suspicious for malignancy in 4 and malignancy in five), providing a sensitivity and a specificity of 9/14 (64%) and 18/18 (100%), respectively. The NGS assay detected somatic mutations in 10/14 malignant and 1/18 benign SGT cases, providing a sensitivity and a specificity of 71% and 94%, respectively.CONCLUSION:The NGS assay may be helpful for detecting the malignant potential in SGT cases and can be used as an ancillary test for SGT cytology. |
| DOI | 10.1111/jop.13109 |
| PMID | 32941702 |