Takayuki Iwamoto    Department   Kawasaki Medical School  Kawasaki Medical School, Department of Breast and Thyroid Surgery,    Position   Assistant Professor
Article types	原著
Language	English
Peer review	Peer reviewed
Title	Microfluidics separation reveals the stem-cell-like deformability of tumor-initiating cells.
Journal	Formal name：Proceedings of the National Academy of Sciences of the United States of America Abbreviation：Proc Natl Acad Sci U S A ISSN code：10916490/00278424
Domestic / Foregin	Foregin
Volume, Issue, Page	109(46),pp.18707-12
Author and coauthor	Weijia Zhang, Kazuharu Kai, Dong Soon Choi, Takayuki Iwamoto, Yen H Nguyen, Helen Wong, Melissa D Landis, Naoto T Ueno, Jenny Chang, Lidong Qin
Publication date	2012/11
Summary	Here we report a microfluidics method to enrich physically deformable cells by mechanical manipulation through artificial microbarriers. Driven by hydrodynamic forces, flexible cells or cells with high metastatic propensity change shape to pass through the microbarriers and exit the separation device, whereas stiff cells remain trapped. We demonstrate the separation of (i) a mixture of two breast cancer cell types (MDA-MB-436 and MCF-7) with distinct deformabilities and metastatic potentials, and (ii) a heterogeneous breast cancer cell line (SUM149), into enriched flexible and stiff subpopulations. We show that the flexible phenotype is associated with overexpression of multiple genes involved in cancer cell motility and metastasis, and greater mammosphere formation efficiency. Our observations support the relationship between tumor-initiating capacity and cell deformability, and demonstrate that tumor-initiating cells are less differentiated in terms of cell biomechanics.
DOI	10.1073/pnas.1209893109
PMID	23112172