Haruyo Yamanishi    Department   Kawasaki Medical School  Kawasaki Medical School, Department of Anatomy,    Position   Assistant Professor
Article types	原著
Language	English
Peer review	Peer reviewed
Title	CoQ10 supplementation elevates the epidermal CoQ10 level in adult hairless mice.
Journal	Formal name：BioFactors (Oxford, England) ISSN code：09516433
Domestic / Foregin	Foregin
Volume, Issue, Page	25,pp.175-8
Author and coauthor	Ashida Y, Yamanishi H, Terada T, Oota N, Sekine K, Watabe K
Publication date	2005
Summary	We have already shown that prolonged supplementation of CoQ(10) in humans reduces the wrinkle area rate and wrinkle volume per unit area in the corner of the eye. CoQ(10) supplementation is known to increase the CoQ(10) level in serum and in many organs; however, the level of CoQ(10) in skin has not yet been fully investigated yet. We examined whether CoQ(10) intake elevates the CoQ(10) and CoQ(9) levels in epidermis, dermis, serum and other organs (kidney, heart, brain, muscle and crystalline lens) in 43-week-old hairless male mice. We also established a method using a high performance liquid chromatograph equipped with an electrochemical detector (HPLC-ECD) to simultaneously quantify CoQ(9) and CoQ(10) in the tissues. CoQ(10) (0, 1, 100 mg/kg p.o.) was administered daily for 2 weeks. CoQ(10) supplementation of 100 mg/kg increased the serum and epidermal CoQ(10) levels significantly, but did not increase the CoQ(10) levels in either dermis or other organs. In conclusion, we showed that CoQ(10) intake elevates the epidermal CoQ(10) level, which may be a prerequisite to the reduction of wrinkles and other benefits related to the potent antioxidant and energizing effects of CoQ(10) in skin.