Haruyo Yamanishi    Department   Kawasaki Medical School  Kawasaki Medical School, Department of Anatomy,    Position   Assistant Professor
Article types	原著
Language	English
Peer review	Peer reviewed
Title	Label-free stimulated Raman scattering microscopy visualizes changes in intracellular morphology during human epidermal keratinocyte differentiation.
Journal	Formal name：Scientific reports ISSN code：20452322
Domestic / Foregin	Foregin
Volume, Issue, Page	9,pp.12601-253
Author and coauthor	Egawa M, Iwanaga S, Hosoi J, Goto M, Yamanishi H, Miyai M, Katagiri C, Tokunaga K, Asai T, Ozeki Y
Publication date	2019/08
Summary	Epidermal keratinocyte (KC) differentiation, which involves the process from proliferation to cell death for shedding the outermost layer of skin, is crucial for the barrier function of skin. Therefore, in dermatology, it is important to elucidate the epidermal KC differentiation process to evaluate the symptom level of diseases and skin conditions. Previous dermatological studies used staining or labelling techniques for this purpose, but they have technological limitations for revealing the entire process of epidermal KC differentiation, especially when applied to humans. Here, we demonstrate label-free visualization of three-dimensional (3D) intracellular morphological changes of ex vivo human epidermis during epidermal KC differentiation using stimulated Raman scattering (SRS) microscopy. Specifically, we observed changes in nuclei during the initial enucleation process in which the nucleus is digested prior to flattening. Furthermore, we found holes left behind by improperly digested nuclei in the stratum corneum, suggesting abnormal differentiation. Our findings indicate the great potential of SRS microscopy for discrimination of the degree of epidermal KC differentiation.