Akira Yamasaki    Department   Kawasaki Medical School  Kawasaki Medical School, Department of Hygiene,    Position   Instructor
Article types	原著
Language	English
Peer review	Peer reviewed
Title	ATM Induces Cell Death with Autophagy in Response to H2O2 Specifically in Caenorhabditis elegans Nondividing Cells.
Journal	Formal name：Oxidative medicine and cellular longevity Abbreviation：Oxid Med Cell Longev ISSN code：19420994/19420994
Domestic / Foregin	Foregin
Volume, Issue, Page	2018,pp.3862070
Author and coauthor	Moriwaki Takahito, Yamasaki Akira, Zhang-Akiyama Qiu-Mei
Authorship	2nd author
Publication date	2018
Summary	Introduction:Ataxia-telangiectasia-mutated (ATM) kinase is a master regulator of the DNA damage response and is directly activated by reactive oxygen species (ROSs) in addition to DNA double-stranded breaks. However, the physiological function of the response to ROSs is not understood.Purpose:In the present study, we investigated how ATM responds to ROSs in Caenorhabditis elegans (C. elegans).Materials and Methods:First, we measured sensitivities of larvae to DNA-damaging agents and ROSs. Next, we analyzed the drug sensitivities of fully matured adult worms, which consist of nondividing somatic cells. Dead cell staining with acridine orange was performed to visualize the dead cells. In addition, we performed GFP reporter assays of lgg-1, an autophagy-related gene, to determine the types of cell death.Results:atm-1(tm5027) larvae showed a wide range of sensitivities to both DNA-damaging agents and ROSs. In contrast, fully matured adult worms, which consist of nondividing somatic cells, showed sensitivity to DNA-damaging agent, NaHSO3, but they showed resistance to H2O2. Dead cell staining and GFP reporter assays of lgg-1 suggest that C. elegans ATM-1 induces the cell death with autophagy in intestinal cells in response to H2O2.Conclusion:We revealed that ATM induces cell death in response to H2O2.
DOI	10.1155/2018/3862070
PMID	30057676