ヤマサキ　アキラ   Akira Yamasaki   山崎　晃    所属   川崎医科大学  医学部 応用医学　衛生学    職種   助教
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	ATM Induces Cell Death with Autophagy in Response to H2O2 Specifically in Caenorhabditis elegans Nondividing Cells.
掲載誌名	正式名：Oxidative medicine and cellular longevity 略　 称：Oxid Med Cell Longev ISSNコード：19420994/19420994
掲載区分	国外
巻・号・頁	2018,pp.3862070
著者・共著者	Moriwaki Takahito, Yamasaki Akira, Zhang-Akiyama Qiu-Mei
担当区分	2nd著者
発行年月	2018
概要	Introduction:Ataxia-telangiectasia-mutated (ATM) kinase is a master regulator of the DNA damage response and is directly activated by reactive oxygen species (ROSs) in addition to DNA double-stranded breaks. However, the physiological function of the response to ROSs is not understood.Purpose:In the present study, we investigated how ATM responds to ROSs in Caenorhabditis elegans (C. elegans).Materials and Methods:First, we measured sensitivities of larvae to DNA-damaging agents and ROSs. Next, we analyzed the drug sensitivities of fully matured adult worms, which consist of nondividing somatic cells. Dead cell staining with acridine orange was performed to visualize the dead cells. In addition, we performed GFP reporter assays of lgg-1, an autophagy-related gene, to determine the types of cell death.Results:atm-1(tm5027) larvae showed a wide range of sensitivities to both DNA-damaging agents and ROSs. In contrast, fully matured adult worms, which consist of nondividing somatic cells, showed sensitivity to DNA-damaging agent, NaHSO3, but they showed resistance to H2O2. Dead cell staining and GFP reporter assays of lgg-1 suggest that C. elegans ATM-1 induces the cell death with autophagy in intestinal cells in response to H2O2.Conclusion:We revealed that ATM induces cell death in response to H2O2.
DOI	10.1155/2018/3862070
PMID	30057676