|
Emi Maruyama
Department Kawasaki Medical School Kawasaki Medical School, Department of Physiology 2, Position Assistant Professor |
|
| Article types | 原著 |
| Language | English |
| Peer review | Peer reviewed |
| Title | Improvement of motor function induced by skeletal muscle contraction in spinal cord-injured rats. |
| Journal | Formal name：The spine journal : official journal of the North American Spine Society Abbreviation：Spine J ISSN code：18781632/15299430 |
| Domestic / Foregin | Foregin |
| Volume, Issue, Page | 19(6),pp.1094-1105 |
| Author and coauthor | Hayashi Norito, Himi Naoyuki, Nakamura-Maruyama Emi, Okabe Naohiko, Sakamoto Issei, Hasegawa Toru, Miyamoto Osamu |
| Publication date | 2019/06 |
| Summary | BACKGROUND:The involvement of neurotrophic factors such as brain-derived neurotrophic factor (BDNF) in functional recovery after spinal cord injury (SCI) by treadmill training has been suggested. The precise mechanism is poorly understood. However, muscle-derived bioactive molecules (myokines) are known to be produced by muscle contraction. Although BDNF is a myokine and is considered to be a potential mediator of neuroplasticity following exercise, its contribution to motor function recovery after SCI has not yet been described in detail.PURPOSE:To investigate the role of muscle contraction in motor function recovery after SCI, with a focus on BDNF.STUDY DESIGN:Male Sprague-Dawley rats (aged 8-9 weeks) were used to establish the SCI model. Percutaneous electrical muscle stimulation (10 mA, 2 Hz, 10 minutes) was applied to both hindlimbs of the rats immediately after SCI. The stimulation was performed once per day for 4 weeks. The sham, SCI only (SCI), and SCI with electrical muscle stimulation (SCI+ES) groups were compared.METHODS:Spinal cord injury was induced by dropping a 20 g rod with an apex diameter of 2 mm from a height of 25 mm onto the spine of an anesthetized rat at the T9 level. Motor function was assessed using the Basso-Beattie-Bresnahan Locomotor Scale, inclined plane test, and rotarod test. One week after injury, terminal deoxynucleotidyl transferase dUTP nick end labeling-positive cells were counted at the injury epicenter, and the level of BDNF was measured in both the spinal cord and the anterior tibial muscle. Four weeks after injury, the cavity volume of the epicenter and the level of phosphorylated growth-associated protein 43 in the spinal cord were measured.RESULTS:Significantly improved Basso-Beattie-Bresnahan scores and inclined plane test results were observed in the SCI+ES group compared with those in the SCI group at 4 weeks post-SCI. We also observed a decrease in the cavity volume and an increase in phosphorylated growth-associated pro |
| DOI | 10.1016/j.spinee.2018.12.012 |
| PMID | 30583107 |