クマノ　イチロウ   Ichiro Kumano   熊野　一郎    所属   川崎医療短期大学  教育部 看護学科    職種   准教授
言語種別	日本語
発表タイトル	ウサギ小腸絨毛上皮のビメンチン陽性細胞 Vimentin positive cells in villus epithelium of the rabbit small intestine.
会議名	第40回日本組織細胞化学会総会
学会区分	全国規模の学会
発表形式	ポスター掲示
講演区分	一般
発表者・共同発表者	Iwatsuki Hirohiko，◎Suda Masumi, kumano Ichiro, Ogawa Chizuru
発表年月日	1999/12/07
開催地 （都市, 国名）	京都
学会抄録	Acta histochemica et cytochemica 32(6),545 1999
概要	In rabbit intestinal epithelium, vimentin intermediate filaments have been reported to be selectively expressed in the M cells of Peyer's patches. When vimentin in rabbit small intestine was stained immunohistochemically in our preliminary study, some vimentin-positive cells were observed in the villus epithelium. In this study, the morphological and histochemical features of vimentin-positive epithelial cells were compared with those of M cells of ileal Peyer's patches. Vimentin was visualized using anti-bovine vimentin monoclonal antibody (Vim3B4) and an avidin-biotin peroxidase complex system. Vimentin-positive cells in the villus epithelium were columnar, and had microvilli shorter than those of adjacent absorptive cells. Vimentin was located from the perinuclear region to the plasma membrane surrounding interepithelial lymphocytes. The alkaline phosphatase activity of the microvilli of the vimentin-positive cells was markedly weak compared with that of adjacent absorptive cells. When glycoconjugate on the apical cell surface was trebly stained with anti-vimentin antibody-alcian blue-PAS, the attenuated brush border of the vimentin-positive epithelial cells was alcian blue-positive and PAS-negative. In contrast, the brush border of the adjacent absorptive cells was clearly alcian blue-negative and PAS-positive. These morphological and histochemical features of the vimentin-positive cells resembled those of M cells. The results suggest that, in addition to M cells, vimentin-positive epithelial cells are also a member of the mucosal immune system in rabbit small intestine.