ニシマツ シンイチロウ
Shinichiro Nishimatsu
西松 伸一郎 所属 川崎医科大学 医学部 一般教養 自然科学 職種 教授 |
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論文種別 | 原著 |
言語種別 | 英語 |
査読の有無 | 査読あり |
表題 | miR-487b, miR-3963 and miR-6412 delay myogenic differentiation in mouse myoblast-derived C2C12 cells |
掲載誌名 | 正式名:BMC cell biology 略 称:BMC Cell Biol ISSNコード:14712121 |
巻・号・頁 | 16(1),pp.13 |
著者・共著者 | Naoki Katase, Kumiko Terada, Takahiro Suzuki, Shin-ichiro Nishimatsu, Tsutomu Nohno |
発行年月 | 2015/04 |
概要 | Background Skeletal muscle differentiation is a multistep, complex pathway in which several important signaling molecules are involved. Recently, microRNAs (miRNAs), endogenous non-coding small RNAs that regulate mRNAs, have been proposed to be involved in skeletal muscle differentiation. In this study, we identified skeletal muscle differentiation-associated miRNAs by comparing miRNA expression profiles between C2C12 cells and Wnt4 over-expressing C2C12 cells (W4-08), which can spontaneously differentiate into myotubes. Results We identified miR-206, miR-133a, and miR-133b as up-regulated miRNAs and miR-487b, miR-3963 and miR-6412 as down-regulated miRNAs in differentiating cells. We focused on the down-regulated miRNAs because their functions were largely unknown. Transfection of mimics of these miRNAs into C2C12 cells resulted in significantly reduced expression of myogenic differentiation markers, including troponin T and myosin heavy chain fast type and slow type, but did not affect the expression of the myogenic transcription factors, MyoD and myogenin. Conclusions These miRNAs were characterized as new myogenic differentiation-associated miRNAs which may delay late myogenic differentiation or maturation. |
DOI | 10.1186/s12860-015-0061-9 |
PMID | 25925429 |