Shinichiro Nishimatsu
Department Kawasaki Medical School Kawasaki Medical School, Department of Natural Sciences, Position Professor |
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Article types | 原著 |
Language | English |
Peer review | Peer reviewed |
Title | Biochemical properties of amphibian bone morphogenetic protein-4 expressed in CHO cells. |
Journal | Formal name:The Biochemical journal Abbreviation:Biochem J ISSN code:02646021/14708728 |
Volume, Issue, Page | 291(2),pp.413-417 |
Author and coauthor | Suzuki Atsushi, Nishimatsu Shin-ichiro, Shoda Akihito, Takebayashi Kimiko, Murakami Kazuo, Ueno Naoto |
Authorship | 2nd author |
Publication date | 1993/04 |
Summary | The biochemical properties of recombinant amphibian bone morphogenetic protein-4 (BMP-4), the cDNA of which has been cloned recently by screening of a Xenopus cDNA library, was characterized. The protein was expressed by the transfection of Chinese hamster ovary (CHO) cells with the cDNA cloned into expression vectors bearing a cytomegalovirus promoter or a simian virus 40 promoter. Northern-blot analysis showed that the latter vector was more efficient for Xenopus BMP-4 expression. Specific antiserum against Xenopus BMP-4 peptide demonstrated that the protein is synthesized as a large precursor, processed to the mature form and then secreted from the cells as a homodimer. Analysis of the biological activity in the conditioned medium revealed that Xenopus BMP-4 has a potent alkaline phosphatase-inducing activity on mouse osteoblastic cells. |
Document No. | 8387268 |