ニシマツ　シンイチロウ   Shinichiro Nishimatsu   西松　伸一郎    所属   川崎医科大学  医学部 一般教養　自然科学    職種   教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Autoinduction of activin genes in early Xenopus embryos.
掲載誌名	正式名：The Biochemical journal 略　 称：Biochem J ISSNコード：02646021/14708728
巻・号・頁	298(2),pp.275-280
著者・共著者	Suzuki Atsushi, Nagai Takeharu, Nishimatsu Shin-ichio, Sugino Hiromu, Eto Yuzuru, Shibai Hiroshiro, Murakami Kazuo, Ueno Naoto
発行年月	1994/03
概要	Activin exhibits a potent mesoderm inducing activity towards the ectodermal tissue (animal cap) of Xenopus laevis blastulae. Thus in order to investigate the role of activin in morphogenesis of early Xenopus embryos, activation of genes for activin A and B was examined by the reverse transcription polymerase chain reaction. In vivo, activin betaB mRNA appears to be present in embryonic stage 1 whereas betaA mRNA is undetectable prior to gastrulation. betaB and betaA mRNAs were noted to accumulate after stages 9 and 15 respectively. Activin gene expression in Xenopus animal caps was examined after treatment with various concentrations of activin A. Under these treatment conditions, both activin betaA and betaB mRNAs accumulated in a dose-dependent fashion after 24 h. The same effect was noted for treatment with similar concentrations of activin B. Accumulation of mRNAs was inhibited by the addition of cycloheximide to the culture medium, consistent with the proposition that activin gene expression requires certain protein factors. In total, therefore, these data suggest that an autoinduction mechanism is involved in the regulation of activin mRNA levels in normal Xenopus embryos and that this mechanism may play a pivotal role during early embryonic development.
文献番号	8135731