ニシマツ　シンイチロウ   Shinichiro Nishimatsu   西松　伸一郎    所属   川崎医科大学  医学部 一般教養　自然科学    職種   教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Isolation and characterization of two alternatively spliced complementary DNAs encoding a Xenopus laevis angiotensin II receptor.
掲載誌名	正式名：Biochimica et biophysica acta 略　 称：Biochim Biophys Acta ISSNコード：00063002
巻・号・頁	1218(3),pp.401-407
著者・共著者	Nishimatsu Shin-ichiro, Koyasu Naoko, Sugaya Takeshi, Ohnishi Junji, Yamagishi Toshiyuki, Murakami Kazuo, Miyazaki Hitoshi
担当区分	筆頭著者
発行年月	1994/08
概要	We have isolated two cDNAs of 1.7 and 3.0 kb, produced by alternative splicing, that encode an angiotensin II (AII) receptor from a Xenopus laevis heart cDNA library. The two clones had identical coding regions with each other and were found to belong to the G protein-coupled receptor superfamily like the mammalian type 1 AII receptors (AT1); their amino acid sequence was 68.7% homologous with the human AT1 receptor sequence. However, there was a 1.3 kb insertion at the 3'-untranslated region of the longer clone. The insertion contained 9 repeats of an ATTTA motif, suggesting that the two mRNAs undergo distinct post-transcriptional regulation by virtue of a difference in their stability. Although the Xenopus receptor exhibited distinct specificities for AII receptor antagonists compared with mammalian AII receptors, several common characteristics, including the effect of dithiothreitol and guanosine 5'-O-(3-thiotriphosphate), demonstrated that the cloned receptor is a counterpart of the mammalian AT1 receptor. Moreover, the cloned receptor was expressed most abundantly in the Xenopus heart, which is inconsistent with the tissue distribution of mammalian AII receptors. This indicated that the Xenopus heart, unlike that of mammals, plays a major role in the AII-dependent regulation of blood pressure and extracellular fluid volume.
DOI	10.1016/0167-4781(94)90193-7
文献番号	7519446