Shinichiro Nishimatsu
Department Kawasaki Medical School Kawasaki Medical School, Department of Natural Sciences, Position Professor |
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Article types | 原著 |
Language | English |
Peer review | Peer reviewed |
Title | Involvement of frizzled-10 in Wnt-7a signaling during chick limb development. |
Journal | Formal name:Development, growth & differentiation Abbreviation:Dev Growth Differ ISSN code:00121592/1440169X |
Volume, Issue, Page | 42(6),pp.561-569 |
Author and coauthor | Kawakami Yasuhiko, Wada Naoyuki, Nishimatsu Shin-ichiro, Nohno Tsutomu |
Publication date | 2000/12 |
Summary | The dorsal ectoderm of the limb bud is known to regulate anterior-posterior patterning as well as dorsalventral patterning during vertebrate limb morphogenesis. Wnt-7a, expressed in the dorsal ectoderm, encodes a key molecule implicated in these events. In the present study, chicken frizzled-10 (Fz-10) encoding a Wnt receptor was used to study mechanisms of Wnt-7a signaling during chick limb patterning, because its expression is restricted to the posterior-distal region of the dorsal limb bud. Fz-10 transcripts colocalize with Sonic hedgehog (Shh) in the dorsal side of stages 18-23 chick limb buds. It was demonstrated that Fz-10 interacts with Wnt-7a to induce synergistically the expression of Wnt-responsive genes, such as Siamois and Xnr3, in Xenopus animal cap assays. In the chick limb bud, Fz-10 expression is regulated by Shh and a signal from the dorsal ectoderm, presumably Wnt-7a, but not by signals from the apical ectodermal ridge. These results suggest that Fz-10 acts as a receptor for Wnt-7a and has a positive effect on Shh expression in the chick limb bud. |
DOI | 10.1046/j.1440-169X.2000.00545.x |
Document No. | 11142678 |