Ichiro Kumano
Department Kawasaki college of Health Professions , Position Associate Professor |
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Article types | 原著 |
Language | English |
Peer review | Peer reviewed |
Title | Ganglion cell differentiation and intermediate filaments in the cervical dorsal root ganglion of the chick embryo. |
Journal | Formal name:Kawasaki Medical Journal ISSN code:03850234 |
Volume, Issue, Page | 28(2.3.4),pp.57-69 |
Author and coauthor | Kumano Ichiro, Iwatsuki Hirohiko, Sasaki Kazunobu |
Authorship | Lead author |
Publication date | 2002 |
Summary | To examine the differentiation processes of cervical dorsal root ganglionic cells, vimentin, nestin and neurofilament-H protein were immunohistochemically detected in chick embryos from stages 15 through 43. During stages 15-17, migrating neural crest cells exclusively expressed vimentin. At stage 20, there was a marked decrease in vimentin, and appearance of nestin in most cells of the ganglionic primordia. However, a few Schwann precursor cells containing only vimentin were also observed. During stages 24-26, spindle-shaped precursors of pseudounipolar neurons containing neurofilament-H protein increased in number in the primordia of the ganglia. During stages 28-30, large round immature neurons containing neurofilament-H protein in both their perikaryon and neurites were recognized. At stage 36, the neurons contained neurofilament-H protein and transiently vimentin. In addition, the precursors of satellite cells, containing neither vimentin, nestin, nor neurofilament-H protein, could be observed around the neurons. At stage 43, three mature cell types could be distinguished; pseudounipolar neurons containing neurofilament-H protein, Schwann cells containing vimentin, and satellite cells containing neither vimentin, nestin, nor neurofilament-H protein. These results clearly demonstrate that differentiation of neural crest cells into neurons, Schwann cells and satellite cells, is reflected by the intermediate filament composition. |