|
Hideho Wada
Department Kawasaki Medical School Kawasaki Medical School, Department of General Medicine, Position Professor with Special Assignment |
|
| Article types | 原著 |
| Language | English |
| Peer review | Peer reviewed |
| Title | Screening for human immunodeficiency virus using a newly developed fourth generation lateral flow immunochromatography assay |
| Journal | Formal name：Journal of Virological Methods Abbreviation：J.Virol.Methods ISSN code：0166-0934 |
| Domestic / Foregin | Foregin |
| Publisher | ELSEVIER |
| Volume, Issue, Page | 274,pp.1-5 |
| Author and coauthor | Itsuhiro Nakagiri, Taizo Tasaka, Miki Okai, Fukue Nakai, Ryoko Bunya, Satomi Nagai, Tomoko Yoshida, Hirotoshi Tokunaga, Eisei Kondo, Hideho Wada |
| Authorship | Last author |
| Publication date | 2019/12 |
| Summary | We screened 17,373 preoperative out patient blood samples using 4th generation lateral flow immunochromatography Daina Screen HIV Combo assay for simultaneously detecting anti-HIV-1/2 and HIV-1 p24 antigen. Of the samples tested, 24 were positive for HIV-1 p24 antigen and 49 for HIV-1/2 antibody. Of the 49, samples, 36 were WB and HIV-1 RNA negative, 10 were WB and HIV-1 RNA positive, and 3 were WB positive, HIV-1 RNA negative, and in-house HIV-1 proviral DNA positive. RT-PCR revealed that of the 24 samples that were p24 antigen positive, one sample was HIV-1 RNA positive, which was reconfirmed using an in-house HIV-1 provirus DNA assay. From the 17,300 HIV-1 p24 antigen and anti-HIV-1/2 negative samples, pools containing 10 negative samples each were tested for HIV-1 by RT-PCR; all results were negative. The Daina Screen HIV Combo assay had a sensitivity and specificity of 100% and 99.7%, respectively, which sufficiently detected HIV infection in the cohort. |
| DOI | 10.1016/j.jviromet.2019.113746 |
| Document No. | 113746 |