マスダ ヨシキ   Yoshiki Masuda
  益田 芳樹
   所属   川崎医療福祉大学  医療福祉学部 臨床心理学科
   職種   特任教授
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 Sponge cytogenetics:Mitotic chromosomes of ten species of freshwater sponge.
掲載誌名 正式名:Zoological Science
略  称:Zool. Sci.
ISSNコード:02890003
巻・号・頁 25(5),480-486頁
著者・共著者 Ishijima Junko, Iwabe Naoyuki, Masuda Yoshiki, Watanabe Yoko, Matsuda Yoichi
発行年月 2008/05
概要 Porifera(sponges) are the most basal phylum of extant metazoabs. To gain insight into sponge genome construction, cytogenetic analysis was performed for ten species of six genera, using conventional Giemsa staining, chromosome banding, and fluorescence in-situ hybridization. The karyotypes were very similar among the ten species, exhibiting a diploid chromosome number of 2n=46 or 48, and usually consisted of microchromosomes with one or two pairs of large chromosomes. The 18S-28S rRNA genes were localized to a single pair of microchromosomes in two Ephydatia species. Hybridization signals of the telomere(TTAGGG)n sequences were observed at the ends of metaphase chromosomes. The genome sizes of Ephydatia fluviatilis and Ephydatia muelleri were estimated by flow cytomeric analysis as about 0.7pg per diploid complement. These freshwater sponge species appear to represent a fairly homogeneous group with respect to karyotypes.
DOI 10.2108/zsj.25.480