マスダ ヨシキ   Yoshiki Masuda
  益田 芳樹
   所属   川崎医療福祉大学  医療福祉学部 臨床心理学科
   職種   特任教授
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 Ribosomal ITS sequences allow resolution of freshwater sponge phylogeny with alignments guided by secondary structure prediction.
掲載誌名 正式名:Journal of molecular evolution
略  称:J Mol Evol
ISSNコード:00222844
巻・号・頁 67(6),608-620頁
著者・共著者 Itskovich Valeria, Gontcharov Andrey, Masuda Yoshiki, Nohno Tsutomu, Belikov Sergey, Efremova Sofia, Meixner Martin, Janussen Dorte
発行年月 2008/12
概要 Freshwater sponges include six extant families which belong to the suborder Spongillina (Porifera). The taxonomy of freshwater sponges is problematic and their phylogeny and evolution are not well understood. Sequences of the ribosomal internal transcribed spacers (ITS1 and ITS2) of 11 species from the family Lubomirskiidae, 13 species from the family Spongillidae, and 1 species from the family Potamolepidae were obtained to study the phylogenetic relationships between endemic and cosmopolitan freshwater sponges and the evolution of sponges in Lake Baikal. The present study is the first one where ITS1 sequences were successfully aligned using verified secondary structure models and, in combination with ITS2, used to infer relationships between the freshwater sponges. Phylogenetic trees inferred using maximum likelihood, neighbor-joining, and parsimony methods and Bayesian inference revealed that the endemic family Lubomirskiidae was monophyletic. Our results do not support the monophyly of Spongillidae because Lubomirskiidae formed a robust clade with E. muelleri, and Trochospongilla latouchiana formed a robust clade with the outgroup Echinospongilla brichardi (Potamolepidae). Within the cosmopolitan family Spongillidae the genera Radiospongilla and Eunapius were found to be monophyletic, while Ephydatia muelleri was basal to the family Lubomirskiidae. The genetic distances between Lubomirskiidae species being much lower than those between Spongillidae species are indicative of their relatively recent radiation from a common ancestor. These results indicated that rDNA spacers sequences can be useful in the study of phylogenetic relationships of and the identification of species of freshwater sponges.
DOI 10.1007/s00239-008-9158-5
文献番号 19009316