Ken Sugimoto    Department   Kawasaki Medical School  Kawasaki Medical School, Department of General Geriatric Medicine,    Position   Professor
Article types	原著
Language	English
Peer review	Peer reviewed
Title	Double deletion of tetraspanins CD9 and CD81 in mice leads to a syndrome resembling accelerated aging.
Journal	Formal name：Scientific reports Abbreviation：Sci Rep ISSN code：20452322/20452322
Domestic / Foregin	Foregin
Volume, Issue, Page	8(1),pp.5145
Author and coauthor	Jin Yingji, Takeda Yoshito, Kondo Yasushi, Tripathi Lokesh P, Kang Sujin, Takeshita Hikari, Kuhara Hanako, Maeda Yohei, Higashiguchi Masayoshi, Miyake Kotaro, Morimura Osamu, Koba Taro, Hayama Yoshitomo, Koyama Shohei, Nakanishi Kaori, Iwasaki Takeo, Tetsumoto Satoshi, Tsujino Kazuyuki, Kuroyama Muneyoshi, Iwahori Kota, Hirata Haruhiko, Takimoto Takayuki, Suzuki Mayumi, Nagatomo Izumi, Sugimoto Ken, Fujii Yuta, Kida Hiroshi, Mizuguchi Kenji, Ito Mari, Kijima Takashi, Rakugi Hiromi, Mekada Eisuke, Tachibana Isao, Kumanogoh Atsushi
Publication date	2018/03
Summary	Chronic obstructive pulmonary disease (COPD) has been recently characterized as a disease of accelerated lung aging, but the mechanism remains unclear. Tetraspanins have emerged as key players in malignancy and inflammatory diseases. Here, we found that CD9/CD81 double knockout (DKO) mice with a COPD-like phenotype progressively developed a syndrome resembling human aging, including cataracts, hair loss, and atrophy of various organs, including thymus, muscle, and testis, resulting in shorter survival than wild-type (WT) mice. Consistent with this, DNA microarray analysis of DKO mouse lungs revealed differential expression of genes involved in cell death, inflammation, and the sirtuin-1 (SIRT1) pathway. Accordingly, expression of SIRT1 was reduced in DKO mouse lungs. Importantly, siRNA knockdown of CD9 and CD81 in lung epithelial cells additively decreased SIRT1 and Foxo3a expression, but reciprocally upregulated the expression of p21 and p53, leading to reduced cell proliferation and elevated apoptosis. Furthermore, deletion of these tetraspanins increased the expression of pro-inflammatory genes and IL-8. Hence, CD9 and CD81 might coordinately prevent senescence and inflammation, partly by maintaining SIRT1 expression. Altogether, CD9/CD81 DKO mice represent a novel model for both COPD and accelerated senescence.
DOI	10.1038/s41598-018-23338-x
PMID	29572511