スギモト　ケン   Ken Sugimoto   杉本　研    所属   川崎医科大学  医学部 臨床医学　総合老年医学    職種   教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Human mannose-binding lectin 2 is directly regulated by peroxisome proliferator-activated receptors via a peroxisome proliferator responsive element.
掲載誌名	正式名：Journal of biochemistry 略　 称：J Biochem ISSNコード：17562651/0021924X
掲載区分	国外
巻・号・頁	154(3),pp.265-273
著者・共著者	Tachibana Keisuke, Takeuchi Kentaro, Inada Hirohiko, Sugimoto Ken, Ishimoto Kenji, Yamashita Masanori, Maegawa Takashi, Yamasaki Daisuke, Osada Shigehiro, Tanaka Toshiya, Rakugi Hiromi, Hamakubo Takao, Sakai Juro, Kodama Tatsuhiko, Doi Takefumi
発行年月	2013/09
概要	Human mannose-binding lectin (MBL) is encoded by the MBL2 gene and is a key player in innate immunity. However, the mechanism of the transcriptional regulation of MBL2 is largely unknown. The peroxisome proliferator-activated receptors (PPARs) are ligand-activated transcription factors that play an important role in a number of biological responses, including lipid homeostasis, immune function and adipogenesis. In this study, we showed that PPARα and PPARγ up-regulate the expression of human MBL2. Using a luciferase assay, electrophoretic mobility-shift assay and chromatin immunoprecipitation assay, we demonstrated that PPARs regulate the expression of human MBL2 via the peroxisome proliferator responsive element (PPRE). On the other hand, MBL2 mRNA expression was not affected by the PPARα ligand both in vivo in rat liver and in vitro in rat H4IIE hepatoma cells. Thus, there is a species difference in regulation of MBL2 gene expression by PPARs between humans and rodents. We also show that the species differences in response to PPAR could be due in part to sequence-specific differences in the PPRE in the promoter region of MBL2. These results indicate that human, but not rat, MBL2 expression is regulated by PPARs via a PPRE.
DOI	10.1093/jb/mvt050
PMID	23711995