Yuta Ishizuka    Department   Kawasaki Medical School  Kawasaki Medical School, Department of Pathophysiology and Metabolism,    Position   Assistant Professor
Article types	原著
Language	English
Peer review	Peer reviewed
Title	AMP-activated protein kinase counteracts brain-derived neurotrophic factor-induced mammalian target of rapamycin complex 1 signaling in neurons.
Journal	Formal name：Journal of neurochemistry Abbreviation：J Neurochem ISSN code：14714159/00223042
Domestic / Foregin	Foregin
Publisher	WILEY
Volume, Issue, Page	127(1),pp.66-77
Author and coauthor	Yuta Ishizuka, Naomasa Kakiya, Lee A. Witters, Noriko Oshiro, Tomoaki Shirao, Hiroyuki Nawa, Nobuyuki Takei
Authorship	Lead author
Publication date	2013/10
Summary	Growth factors and nutrients, such as amino acids and glucose, regulate mammalian target of rapamycin complex 1 (mTORC1) signaling and subsequent translational control in a coordinated manner. Brain-derived neurotrophic factor (BDNF), the most prominent neurotrophic factor in the brain, activates mTORC1 and induces phosphorylation of its target, p70S6 kinase (p70S6K), at Thr389 in neurons. BDNF also increases mammalian target of rapamycin-dependent novel protein synthesis in neurons. Here, we report that BDNF-induced p70S6K activation is dependent on glucose, but not amino acids, sufficiency in cultured cortical neurons. AMP-activated protein kinase (AMPK) is the molecular background to this specific nutrient dependency. Activation of AMPK, which is induced by glucose deprivation, treatment with pharmacological agents such as 2-deoxy-D-glucose, metformin, and 5-aminoimidazole-4-carboxamide ribonucleoside or forced expression of a constitutively active AMPKα subunit, counteracts BDNF-induced phosphorylation of p70S6K and enhanced protein synthesis in cortical neurons. These results indicate that AMPK inhibits the effects of BDNF on mTORC1-mediated translation in neurons.
DOI	10.1111/jnc.12362
PMID	23841933