イシヅカ ユウタ   Yuta Ishizuka
  石塚 佑太
   所属   川崎医科大学  医学部 基礎医学 病態代謝学
   職種   講師
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 Leucine induces phosphorylation and activation of p70S6K in cortical neurons via the system L amino acid transporter.
掲載誌名 正式名:Journal of neurochemistry
略  称:J Neurochem
ISSNコード:14714159/00223042
掲載区分国外
出版社 WILEY
巻・号・頁 106(2),934-42頁
著者・共著者 Yuta Ishizuka, Naomasa Kakiya, Hiroyuki Nawa, Nobuyuki Takei
発行年月 2008/07
概要 p70S6 kinase is a multipotent kinase that phosphorylates substrates in response to extracellular stimuli. This kinase activity inhibits apoptosis, regulates cell size and controls translation. In the CNS, p70S6K also participates in synaptic plasticity. In this study, we report that leucine, a branched-chain amino acid, induces phosphorylation and activation of p70S6 kinase in cortical neurons. Leucine also induces phosphorylation of S6 protein, a substrate of p70S6K. These effects of leucine are completely inhibited by rapamycin, consistent with mammalian target of rapamycin mediating p70S6 phosphorylation. Finally, we demonstrate that the action of leucine on cortical neurons is mediated by the system L amino acid transporter. Neurons express components of system L amino acid transporter LAT1, LAT2, and CD98. Leucine uptake and its effect on p70S6 kinase are both inhibited by a specific inhibitor of system L amino acid transporter. We propose that leucine plays important roles in regulating signaling by p70S6 kinase by acting as an intercellular communicator in the CNS.
DOI 10.1111/j.1471-4159.2008.05438.x
PMID 18435829