ハヤシ　シュウイチ   Shuichi Hayashi   林　周一    所属   川崎医科大学  医学部 基礎医学　解剖学    職種   准教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Mechanism of flagellar oscillation: Bending-induced switching of dynein activity in elastase-treated axonemes of sea urchin sperm.
掲載誌名	正式名：Journal of Cell Science
掲載区分	国外
巻・号・頁	121(7),pp.2833-2843
著者・共著者	Hayashi, S. and Shingyoji, C.
担当区分	筆頭著者
発行年月	2008
概要	Oscillatory movement of eukaryotic flagella is caused by dynein-driven microtubule sliding in the axoneme. The mechanical feedback from the bending itself is involved in the regulation of dynein activity, the main mechanism of which is thought to be switching of the activity of dynein between the two sides of the central pair microtubules. To test this, we developed an experimental system using elastase-treated axonemes of sperm flagella, which have a large Ca(2+)-induced principal bend (P-bend) at the base. On photoreleasing ATP from caged ATP, they slid apart into two bundles of doublets. When the distal overlap region of the slid bundles was bent in the direction opposite to the basal P-bend, backward sliding of the thinner bundle was induced along the flagellum including the bent region. The velocity of the backward sliding was significantly lower than that of the forward sliding, supporting the idea that the dynein activity alternated between the two sides of the central pair on bending. Our results show that the combination of the direction of bending and the conformational state of dynein-microtubule interaction induce the switching of the dynein activity in flagella, thus providing the basis for flagellar oscillation.