Kazuhito Tsuboi    Department   Kawasaki Medical School  Kawasaki Medical School, Department of Pharmacology,    Position   Associate Professor
Article types	原著
Language	English
Peer review	Peer reviewed
Title	Lysophospholipase D activity on oral mucosa cells in whole mixed human saliva involves in production of bioactive lysophosphatidic acid from lysophosphatidylcholine.
Journal	Formal name：Prostaglandins & Other Lipid Mediators Abbreviation：Prostaglandins Other Lipid Mediat ISSN code：10988823/10988823
Domestic / Foregin	Foregin
Volume, Issue, Page	174,pp.106881
Author and coauthor	Tsutsumi T, Taira S, Matsuda R, Kageyama C, Wada M, Kitayama T, Morioka N, Morita K, Tsuboi K, Yamazaki N, Kido J, Nagata T, Dohi T, Tokumura A
Publication date	2024/08/10
Summary	We reported that lysophosphatidic acid (LPA) is present at 0.8 μM in mixed human saliva (MS). In this study, we examined the distribution, origin, and enzymatic generation pathways of LPA in MS. LPA was distributed in the medium and cell pellet fraction; a true level of soluble LPA in MS was about 150 nM. The soluble LPA was assumed to be generated by ecto-type lysophospholipase D on exfoliated cells in MS from LPC that originated mainly from the major salivary gland saliva. Our results with the albumin-back extraction procedures suggest that a significant pool of LPA is kept in the outer layer of the plasma membranes of detached oral mucosal cells. Such pool of LPA may contribute to wound healing in upper digestive organs including oral cavity. We obtained evidence that the choline-producing activity in MS was mainly due to Ca2+-activated lysophospholipase D activity of glycerophosphodiesterase 7.
DOI	10.1016/j.prostaglandins.2024.106881
PMID	39134206